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作者用从细粒棘球绦虫中分离得到的DNA建立了一个小基因组DNA库,并鉴定及分离了带有细粒棘球绦虫DNA序列的几株重组质粒。从寄生于人体的细粒棘球蚴中分离得到DNA,用内切酶Sau 3A消化并在0.8％琼脂上电泳,分离到0.5—5.0kb大小的DNA片段,此片断与经Bam H1内切酶切割后经磷酸酶处理的线状载体质粒pAF153,在T_4DNA连接酶的作用下进行连接,形成含细粒棘球绦虫DNA插入序列的重组质粒。将
The authors established a small genomic DNA library using DNA isolated from Echinococcus granulosus and identified and isolated several recombinant plasmids containing the DNA sequence of Echinococcus granulosus. DNA was isolated from the echinococcus granulosus parasitic on the human body, digested with the endonuclease Sau 3A and electrophoresed on 0.8% agar, and a DNA fragment of 0.5-5.0 kb in size was isolated. This fragment was digested with Bam H1 endonuclease After cleavage, the phosphatase-treated linear vector plasmid pAF153 was ligated under the action of T_4 DNA ligase to form a recombinant plasmid containing the Echinococcus granulosus DNA insert. will