论文部分内容阅读
本文报告了西非冈比亚高疟区的大批居民中有抗恶性疟子孢子期的抗体。疟疾传播高峰期后2～3个月,从恶性疟高疟区的4个年龄组(5～9岁,10～15岁,20～49岁和50岁以上)的居民采集158份血清样本,对活的和经戊二醛固定的恶性疟子孢子进行了试验。以实验室饲养的冈比亚按蚊喂饲每立方毫升含50～3,000个恶性疟配子体的患者血液,感染后12～17天解剖唾液腺,收集的子孢子保存在冷的组织培养基199中。用0. 1%戊二醛溶液在室温下对子孢子固定10分钟,用于子孢子沉淀试验(CSP)和间接免疫荧光试验,来检测子孢子抗体。
This article reports the presence of anti-malaria sporozoite antibodies in a large population of residents in the high malaria region of Gambia, Western Africa. Two to three months after the peak of malaria transmission, 158 serum samples were collected from residents of four age groups (5-9 years old, 10-15 years old, 20-49 years old and 50 years of age or older) in the P. falciparum malaria area, Live and glutaraldehyde-fixed P. falciparum spores were tested. Salmon glands were dissected 12 to 17 days after infection in a laboratory culture of Anopheles gambiae fed the blood of patients with 50 to 3,000 falciparum malaria gametocytes per cubic milliliter and the collected sporozoites were stored in cold tissue culture media 199. Sporozoites were fixed with 0.1% glutaraldehyde solution for 10 minutes at room temperature for sporozoite precipitation assay (CSP) and indirect immunofluorescence assay to detect sporozoite antibodies.