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目的:研究热痛方预处理对大鼠急性肺损伤的保护作用及其机制。方法:40只健康成年SD雄性大鼠,随机分为4组:生理盐水组(NS组)、模型组(ALI组)、热痛方组(RT组)和地塞米松组(DM组)。除NS组外,每组大鼠尾iv脂多糖(LPS5 mg.kg-1)复制急性肺损伤模型,并于注射LPS 2 h后行腹腔麻醉,腹主动脉采血处死。测肺系数、外周血白细胞与血小板数,行肺泡灌洗后测灌洗液中的白细胞数,ELISA法检测灌洗液中肿瘤坏死因子α(TNF-α)的含量,并在光镜下观察肺组织形态学变化。结果:应用热痛方预先给药可明显减轻内毒素引起的肺组织形态学改变,与ALI组相比,RT组肺系数、肺泡灌洗液中白细胞数及TNF-α含量均显著降低(P<0.01或P<0.05)、外周血白细胞数升高(P<0.05);与DM组相比,肺系数、外周血小板计数、肺泡灌洗液中白细胞数也存在差异。结论:热痛方预先给药可能通过减轻肺部的炎性渗出、改善肺部的局部微循环、抑制炎症因子TNF-α的产生等途径减轻内毒素引起的急性肺损伤。
Objective: To study the protective effects and mechanism of heat-tong preconditioning on acute lung injury in rats. Methods: Forty healthy male SD rats were randomly divided into 4 groups: NS group, ALI group, RT group and DM group. Except NS group, acute lung injury model was induced by lipopolysaccharide (LPS5 mg.kg-1) from the caudal vein of each group and celiac anesthesia was performed 2 h after LPS injection. Lung coefficient, peripheral blood leukocytes and platelets, alveolar lavage were measured after the number of leukocytes in the lavage fluid, ELISA assay lavage fluid tumor necrosis factor α (TNF-α) content, and observed by light microscopy Lung tissue morphological changes. Results: Pretreatment with heat pain prescription could obviously reduce endotoxin-induced morphological changes of lung tissue. Compared with ALI group, lung coefficient, leukocyte count and TNF-α in bronchoalveolar lavage fluid were significantly decreased (P <0.01 or P <0.05), and the number of leukocytes in peripheral blood increased (P <0.05). Compared with DM group, pulmonary coefficient, peripheral platelet count and leukocyte count in bronchoalveolar lavage fluid were also different. Conclusion: Pretreatment with heat Prescription can relieve endotoxin-induced acute lung injury by relieving pulmonary inflammatory exudation, improving local microcirculation and inhibiting the production of inflammatory cytokine TNF-α.