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本文研究了土壤干旱胁迫和PEG渗透胁迫对苗木叶片脯氨酸和可溶性糖含量的影响,并讨论了脯氨酸和可溶性糖与渗透调节的关系.结果表明:①土壤干旱胁迫使落叶松前叶片大量累积脯氨酸,表现出脯氨酸的增加与水势的下降呈显著负相关.与落叶松不同,樟子松苗木并不累积脯氨酸.PEG渗透胁迫处理无论是落叶松还是樟子松苗木都累积脯氨酸.樟子松苗木在两种处理中表现出的不同规律可能是由于我们研究的水分亏缺程度和苗木年龄不同造成的.②无论是土壤干旱胁迫还是渗透胁迫,在一定胁迫范围内,随着胁迫的发展,叶片可溶性糖增加;当进一步胁迫时,可溶性糖含量随之下降.脯氨酸和可溶性糖含量的上述变化是苗木对水分胁迫的一种适应.研究表明:脯氨酸和可溶性糖在苗木的渗透调节中起重要作用.
In this paper, the effects of soil drought stress and PEG osmotic stress on the contents of proline and soluble sugar in seedling leaves were studied, and the relationship between proline and soluble sugar and osmotic adjustment was discussed.The results showed that: (1) Accumulation of proline, proline showed an increase and decreased water potential was significantly negative correlation.Compared with the larch, Pinus sylvestris seedlings did not accumulate proline.PEG osmotic stress treatment, whether larch or Pinus sylvestris seedlings All accumulate proline.The different rules of Pinus sylvestris seedlings in the two treatments may be due to the degree of water deficit and age of seedlings in our study.② Whether soil drought stress or osmotic stress, Within the range, the soluble sugar content increased with the development of stress, and the soluble sugar content decreased with further stress.The above changes of proline and soluble sugar content were the adaptation of seedling to water stress.The results showed that: Amino acids and soluble sugars play an important role in osmotic adjustment of seedlings.