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目的 :探讨复制 SD大鼠抗 Thy1.1系膜增生性肾炎实验模型的方法 ,观察其临床病理特点和肾小球系膜区细胞增殖核抗原 (PCNA)、凋亡调节基因 Bcl- 2的表达。方法 :用 SD大鼠胸腺细胞免疫新西兰白兔 ,制备兔抗大鼠胸腺细胞免疫血清 (Anti- Thy1.1serum,ATS) ,再将 ATS经尾静脉注射给 SD大鼠 ,诱导其发生系膜增生性肾炎(Ms PGN)。每日检测尿蛋白 ,用 HE和 PASM染色法观察肾小球系膜增生程度 ,用免疫组化 L SAB法检测系膜区 PCNA及 Bcl- 2的表达。结果 :ATS效价可达 1∶ 32 0 0 ;诱导大鼠 Ms PGN的 ATS最适用量为 :0 .0 1m L/ g体重 ;大鼠 Ms PGN的临床特点为大量蛋白尿 ;病理特点为肾小球系膜细胞增生 ;增生期系膜区 PCNA、Bcl- 2的表达增高。结论 :用 ATS可诱导典型的、稳定的大鼠 Ms PGN实验模型 ;免疫反应和细胞凋亡受抑制参与了肾小球系膜增生的发病机制。
OBJECTIVE: To investigate the clinical and pathological characteristics of SD rat model of anti-Thy1.1 mesangial proliferative glomerulonephritis and to observe the expression of proliferating cell nuclear antigen (PCNA) and apoptosis-regulating gene Bcl-2 in glomerular mesangial area . Methods: New Zealand white rabbits were immunized with SD rat thymocytes to prepare rabbit anti-Thy1.1 serum (ATS), and then ATS was injected into SD rats via the tail vein to induce mesangial hyperplasia Nephritis (Ms PGN). Urine protein was detected daily. The degree of mesangial proliferation was observed by HE and PASM staining. The expression of PCNA and Bcl-2 was detected by immunohistochemical L SAB method. Results: The titer of ATS was 1:32 0 0. The optimal dose of ATS for inducing rat PGNP was 0. 01 m L / g body weight. The clinical features of Ms PGN in rats were massive proteinuria. The pathological features were kidney Glomerular mesangial cell proliferation; hyperplasia mesangial area PCNA, Bcl-2 expression increased. CONCLUSION: ATS can induce a typical and stable model of Ms PGN in rats. Inhibition of immune response and apoptosis is involved in the pathogenesis of mesangial proliferation.