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目的探讨肿瘤坏死因子-α(TNF-α)基因-863、-857、-238三个位点多态性与安徽地区汉族人Graves病(GD)发病易感性的关系。方法采用聚合酶链反应-序列特异性引物(PCR-SSP)方法,检测和比较254例GD患者和212例正常对照者的TNF-α-863、-857、-238位点基因型和等位基因的频率。结果 TNF-α-863位点A等位基因GD组频率(16.73%)高于正常对照组(11.79%),P<0.05,OR=1.503;AA+CA基因型频率GD组(32.68%)明显高于正常对照组(23.58%),P<0.05,OR=1.573。TNF-α-857、-238两位点基因型及等位基因频率在两组间比较差异无显著性。将GD组按性别分层后比较,-863、-857、-238三个位点的基因型及等位基因频率在不同性别间差异均无显著性。结论 TNF-α-863位点基因多态性与安徽地区汉族人GD发病有相关性,而-857、-238位点基因多态性与安徽地区汉族人GD发病无明显相关性。
Objective To investigate the relationship between the polymorphisms of -863, -857 and -238 of tumor necrosis factor-α (TNF-α) gene and the susceptibility to Graves’ disease (GD) in Han nationality in Anhui province. Methods The genotypes and alleles of TNF-α-863, -857 and -238 in 254 patients with GD and 212 healthy controls were detected and compared by polymerase chain reaction-sequence specific primers (PCR-SSP) The frequency of the gene. Results The frequency of A allele in TNF-α-863 group (16.73%) was higher than that in normal control group (11.79%), P <0.05, OR = 1.503. The frequency of AA + CA genotype was significantly higher in GD group (32.68% Higher than the control group (23.58%), P <0.05, OR = 1.573. TNF-α-857, -238 two loci genotype and allele frequencies in the two groups showed no significant difference. According to gender stratification in GD group, the genotype and allele frequencies of -863, -857 and -238 were not significantly different among different genders. Conclusion The polymorphism of TNF-α-863 locus is related to the incidence of GD in Han nationality in Anhui Province. However, there is no significant correlation between the -857 and -238 locus polymorphism and the incidence of GD in Han nationality in Anhui Province.