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目的:研究CD40和NF-κB在子痫前期患者组的脐静脉内皮细胞(HUVEC)中的表达,探讨其在子痫前期发病过程中的作用。方法:应用子痫前期患者组的血清冲击人HUVEC株,电镜下观察HUVEC的形态变化,流式细胞仪下测定细胞的凋亡,用RT-PCR方法检测HUVEC中CD40和NF-κB的表达情况。结果:1电镜下正常孕妇组HUVEC呈“铺路石”状单层镶嵌排列,子痫前期组HUVEC形态呈典型损伤改变,细胞稀疏,细胞核与细胞浆分界模糊不清,胞浆中有暗色颗粒。2流式细胞仪测定子痫前期组HUVEC的细胞凋亡较正常组明显升高,差异有显著意义(P<0.05);3 RT-PCR测定显示子痫前期患者组HUVEC中CD40m RNA及NF-Bm RNA水平均较正常组明显升高,差异有显著意义(P<0.05);4子痫前期组及对照组的CD40和NF-k B表达均呈显著性正相关。结论:CD40和NF-κB的激活均参与了子痫前期的发病机制,并在其中发挥重要作用。
Objective: To investigate the expression of CD40 and NF-κB in human umbilical vein endothelial cells (HUVEC) in patients with preeclampsia and to explore its role in the pathogenesis of preeclampsia. Methods: The serum of human preeclampsia patients was challenged with human HUVEC. The morphological changes of HUVEC were observed under electron microscope. The apoptosis of HUVECs was detected by flow cytometry. The expression of CD40 and NF-κB in HUVECs were detected by RT-PCR . Results: 1 Under the electron microscope, HUVEC in normal pregnant women showed “” paving stone "monolayer inlaid arrangement. The morphology of HUVEC in preeclampsia group showed typical damage, the cells were sparse, the nucleus and cytoplasm demarcation were blurred, and the cytoplasm was dark Granules. The results of flow cytometry showed that the apoptosis rate of HUVEC in preeclampsia group was significantly higher than that in normal group (P <0.05); 3 RT-PCR showed that the expression of CD40mRNA and NF- Bm RNA levels were significantly higher than the normal group, the difference was significant (P <0.05); 4 preeclampsia group and control group of CD40 and NF-k B expression was significantly positive correlation. Conclusion: The activation of both CD40 and NF-κB is involved in the pathogenesis of preeclampsia and plays an important role.