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油橄榄离体茎培养出根已有报道,但无诱导完整植株的试验结果。我们以油橄榄下胚轴、子叶、胚根、嫩茎、茎尖和叶片等作为外植体进行了试验,除从茎尖诱导发生小植株外(待发表),在下胚轴上也已诱导出完整植株。从甘蓝下胚轴诱导发生小植株已有报道,本文就油橄榄下胚轴诱导完整植株作简要报道。油橄榄(Olea europaea L.)种子经消毒处理后,通过无菌培养得到无菌苗。在幼苗下胚轴伸长到2—3厘米时,在无菌条件下切除茎尖、子叶
Olive root explants cultured in vitro have been reported, but without inducing the test results of intact plants. We tested olive root hypocotyls, cotyledons, radicles, tender stems, shoot tips and leaves as explants. Except for plantlets induced from shoot tips (to be published), we also induced them on the hypocotyls Complete plant. Induction of plantlets from the hypocotyls of cabbage has been reported, and the brief report on intact plants induced by the hypocotyls of Olive was carried out. After the seeds of Olea europaea L. were sterilized, the aseptic seedlings were obtained by aseptic culture. In the seedling hypocotyl elongation to 2-3 cm, under aseptic conditions excision of the shoot tip, cotyledons