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目的胃癌的发生与发展中有细胞凋亡的变化，叶酸抗肿瘤的机理涉及到维持ＤＮＡ甲基化水平等，其与凋亡的关系尚未明了。方法以高低两种浓度的叶酸干预培养的人胃癌ＭＫＮ－４５和ＭＫＮ－２８细胞系７２小时后，以原位ＤＮＡ断端切口标记法和ＤＮＡ琼脂糖凝胶电泳检测凋亡形态学改变和ＤＮＡ断裂情况。结果未加干预者两种细胞系的凋亡指数均低于５％，不同分化细胞系间比较差异无显著性；低浓度叶酸可诱导ＭＫＮ－４５细胞凋亡，而高浓度者使ＭＫＮ－２８细胞凋亡率增加。结论胃癌细胞自然凋亡率较低。叶酸干预可影响凋亡的发生。
Objective The occurrence and development of gastric cancer have changes of apoptosis. The mechanism of folic acid anti-tumor involves in maintaining DNA methylation level, and the relationship between apoptosis and apoptosis is not yet clear. Methods 72 hours after culturing the human gastric cancer MKN-45 and MKN-28 cell lines with different concentrations of folic acid, the morphological changes of apoptosis and DNA were detected by in situ DNA nick end labeling and DNA agarose gel electrophoresis Fracture situation. Results The apoptotic index of both cell lines was lower than 5% with no intervention, and there was no significant difference among different differentiated cell lines. Low concentrations of folic acid induced the apoptosis of MKN-45 cells, while the high concentrations of MKN-28 The rate of apoptosis increased. Conclusion The natural apoptosis rate of gastric cancer cells is low. Folic acid intervention can affect the occurrence of apoptosis.