论文部分内容阅读
本文作者报道应用多聚酶链反应(PCR)测定斑点杂交和 Southern 杂交阴性的慢性乙型肝炎患儿血清和肝组织的 HBV DNA。材料和方法作者对慢性乙型肝炎患儿的216份血清和104例肝活检标本用斑点杂交和Southern 杂交法测定 HBV DNA。87份血清用斑点法未能测出病毒序列。40例患儿 Southern 法阴性,无1例肝组织证实有 HBV DNA 整合。患儿年龄为1.5～18(平均12.8)岁。40例中9例组织学诊断为慢性活动性肝炎,15例慢性迁延性肝炎,9例轻度肝炎。2例为肝衰竭伴大块性肝坏死,5例为 HBsAg 健康携带者。用PCR 测定 HBV DNA。同时并检测血清谷草转氨酶(AST)、谷丙转氨酶(ALT)及 HBsAg、H]BeAg、抗-HBe。
The authors report the use of polymerase chain reaction (PCR) to detect HBV DNA in serum and liver tissue of children with chronic hepatitis B who are negative for dot hybridization and Southern hybridization. Materials and Methods HBV DNA was determined by dot blot hybridization and Southern blotting in 216 serum samples and 104 liver biopsies from children with chronic hepatitis B. 87 serums failed to detect the virus sequence by the dot blot method. 40 cases of Southern patients with negative, no cases of liver tissue confirmed HBV DNA integration. Children aged 1.5 to 18 (average 12.8) years old. Nine of 40 patients were diagnosed as chronic active hepatitis, 15 chronic persistent hepatitis and 9 mild hepatitis. 2 cases of liver failure with massive liver necrosis, 5 cases of HBsAg healthy carriers. HBV DNA was determined by PCR. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT) and HBsAg, H] BeAg and anti-HBe were also detected.