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近几年随着免疫细胞研究的不断深入,对“乳斑”性质、性差等研究,渐受重视。本文对大鼠“乳斑”的结构和性激素的关系进行了探讨。 1 材料与方法 1.1 制片及镜检 成龄SD大鼠16只,雌雄各半,乙醚吸入麻醉,沿胃大弯取下大网膜,制铺片,10%甲醛固定,1%苏木精(H)单染,镜下数完整大网膜乳斑的总数,用目镜测微仪随机检测每鼠不同部位50个“乳斑”的长径(A)和短径(B),按D=
In recent years, with the continuous deepening of the study of immune cells, the research on the nature and poor sex of “breast spot” has gained increasing attention. This article discusses the relationship between the structure of rats’ “breast spot” and sex hormones. 1 Materials and methods 1.1 Preparation and microscopy 16 adult SD rats, male and female, ether inhalation anesthesia, take a large omentum along the greater curvature of the stomach, preparation of the preparation, 10% formaldehyde, 1% hematoxylin (H) Single staining, the total number of complete omental milk spots under microscopy, randomly measuring the long (A) and short (B) diameters of 50 “sweet spots” in different parts of each mouse using an eyepiece micrometer. =