【摘 要】
：
In the present study, a total of 24 Mabs were produced against bluetongue virus (BTV) by polyethyleneglycol (PEG) mediated fusion method using sensitized lympho
【机 构】
：
Division of Virology, Indian Veterinary Research Institute, Mukteswar 263 138, Nainital (Distt.) Utt
论文部分内容阅读
In the present study, a total of 24 Mabs were produced against bluetongue virus (BTV) by polyethyleneglycol (PEG) mediated fusion method using sensitized lymphocytes and myeloma cells. All these clones were characterized for their reactivity to whole virus and recombinant BTV-VP7 protein, titres, isotypes and their reactivity with 24 BTV-serotype specific sera in cELISA. Out of 24 clones, a majority of them (n = 18)belong to various IgG subclasses and the remaining (n = 6) to the IgM class. A panel of eight clones reactive to both whole BTV and purified rVP7 protein were identified based on their reactivity in iELISA. For competitive ELISA, the clone designated as 4A10 showed better inhibition to hyperimmune serum of BTV serotype 23. However, this clone showed a variable percent of inhibition ranging from 16.6% with BTV 12 serotype to 78.9% with BTV16 serotype using 24 serotype specific sera of BTV originating from guinea pig at their lowest dilutions. From the available panel of clones, only 4A 10 was found to have a possible diagnostic application.
其他文献
分析了自治混沌系统的投影同步问题.基于线性系统的稳定判定准则,提出一种新的线性分离的同步方法,并采用该方法实现了Lorenz系统,R(o)ssler系统和超混沌Chen系统的投影同步.
文中提出并实现了一种基于点对点通信、按功能组织的分层分布双总线通信方案。通过引入镜像节点、总线负荷均衡等技术,使该方案可灵活高效地实现分布自动化功能和信息局部整
2003年,美国预防、检测、评估与治疗高血压全国联合委员会第七次报告(JNC 7)[1]将收缩压120～139 mmHg和/或舒张压80～89 mmHg定义为高血压前期.根据我国国情,2005年中国高血压防
In this paper, the k-ε turbulence model is usedto simulate the three-dimensional turbulence flow over thestepped spillway at the Yubeishan reservoir. In order
原发性高血压是以血压升高为主要临床表现的综合症，通常简称高血压，它是多种心、脑血管疾病的重要病因和危险因素。临床上以头晕、头痛、耳鸣、疲劳、健忘、血压升高为主要表现。由于人们的饮食和生活习惯改变，近年，发病率呈明显上升趋势，且发病年龄年轻化，并可引起心脑、肾等重要脏器的损害。因此，应积极防控、治疗。
目的 探讨阿昔洛韦过量导致急性肾功能衰竭的组织学特点、临床表现、治疗以及预后.方法 报告1例阿昔洛韦过量导致的急性肾功能衰竭患者的临床、病理及预后资料,系统复习相关
对2010年中国科技资源管理论坛进行综述,介绍我国科技条件资源管理的最新理论研究成果与实践经验,探讨“十二五”时期我国科技条件资源管理的基本方向.
以GaP为靶材采用射频磁控溅射法制备GaP红外光学薄膜,通过保持ArⅠ750nm发射光谱线强度不变获得了不同工艺参数,并对沉积过程进行了计算机模拟.功率较小、气压较大时,Ga和P的
校企导学制高技能创新人才培养是在总结校企合作实践经验基础上提出来的,通过引入企业技术人员实际参与教学过程,结合项目实施,强化实际工作过程培养,强化师生的实践能力与创
为了找出多环芳烃生成二噁英的反应途径,选取了多环芳烃中具有代表性、和二噁英结构比较类似的芴作为多环芳烃的模型化合物,以氯化铜和石英砂作为模拟飞灰的基本成分,比较添