论文部分内容阅读
目的 探讨细胞周期调控基因ｐ２７ｋｉｐｌ在膀胱移行细胞癌中表达及与临床病理特征之间的关系．方法 应用鼠抗人ｐ２７ｋｉｐｌ单克隆抗体对６５例膀胱移行细胞癌（ＢＴＣＣ）和１２例正常粘膜进行免疫组化ＳＰ法染色，观察ｐ２７ｋｉｐｌ的表达情况。结果ｐ２７ｋｉｐｌ在ＢＴＣＣ和正常膀胱粘膜中的阳性表达率分别为３２．３％和７５．０％，ＢＴＣＣ显著低于正常膀胱粘膜（Ｐ＜０．０１）；ｐ２７ｋｉｐｌ在ＢＴＣＣ中表达随着临床分期和病理分级的增高而降低（Ｐ＜０．０５），有淋巴结转移组显著低于无淋巳结转移组（Ｐ＜０．０５）。结论 ｐ２７ｋｉｐｌ对ＢＴＣＣ的分化、浸润和转移可能起抑制作用。检测ＢＴＣＣ中ｐ２７ｋｉｐｌ表达对ＢＴＣＣ的生物学行为评价和判断预后具有重要意义．
Objective To investigate the expression of p27kipl in bladder transitional cell carcinoma and its relationship with clinicopathological features. Methods The anti-human p27kipl monoclonal antibody was used to detect the expression of p27kipl in 65 cases of bladder transitional cell carcinoma (BTCC) and 12 cases of normal mucosa by immunohistochemistry. Results The positive rates of p27kipl in BTCC and normal bladder mucosa were 32.3% and 75.0%, respectively, and BTCC was significantly lower than that in normal bladder mucosa (P <0.01). The expression of p27kipl in BTCC was positively correlated with clinical stage and Pathological grade increased and decreased (P <0.05), with lymph node metastasis was significantly lower than without lymph node metastasis (P <0.05). Conclusion p27kipl may inhibit the differentiation, invasion and metastasis of BTCC. Detection of p27kipl expression in BTCC has important implications for the evaluation of the biological behavior of BTCC and prognosis.