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目的设计一种能快速简便测定尿碘的尿碘测定仪。方法以砷铈催化动力曲线法为本仪器测定方法,尿样按WS/T 107.1—2016方法消化,在420 nm下以1 s的频率扫描100 s记录催化动力曲线,以log A-t曲线的斜率来定量。设计了新型尿碘测定仪的结构光学系统及软件界面。结果本尿碘测定仪在尿碘浓度为0μg/L~1 000μg/L时线性关系良好;方法的检出限为12μg/L;考察高、中、低3个浓度的加标样品,加标回收率在93.9%~108.7%,相对标准差(RSD)为0.32%~2.04%;对20份尿样分别采用催化动力曲线法和WS/T 107.1—2016 2种方法测定其碘含量,测定结果差异无统计学意义(P>0.05)。结论此尿碘测定仪测定尿碘准确、快速,样品消化后2 min内可完成分析,适合用于尿碘的快速测定。
Objective To design a urinary iodine analyzer for rapid and simple determination of urinary iodine. Methods Arsenic and cerium catalytic kinetic curve method for the determination of the instrument, the urine samples were digested WS / T 107.1-2016 method, at 420 nm at a scanning frequency of 1 s 100 s record catalytic kinetic curve to log At curve slope to Quantitative. Design a new type of urinary iodine detector structure of the optical system and software interface. Results The urinary iodine analyzer had a good linearity when the urinary iodine concentration was 0μg / L ~ 1 000μg / L. The detection limit of this method was 12μg / L. The spiked samples with high, medium and low concentrations were spiked The recoveries ranged from 93.9% to 108.7% and the relative standard deviations (RSDs) ranged from 0.32% to 2.04%. The urinary contents of 20 samples were determined by catalytic kinetic curve and WS / T 107.1-2016, respectively. The difference was not statistically significant (P> 0.05). Conclusion The urinary iodine determination of urine iodine accurate and rapid, digestion within 2 min after the sample can be completed for analysis, suitable for rapid determination of urinary iodine.