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本文报道用血清学方法,鉴别获自现场蚊虫中的子孢子种类。抗原制备:按Pacheco等(1979)方法,将新杀死或冰冻保存的蚊子,经不连续的梯度高速离心,分离子孢子,并以子孢子50～150/μl的浓度悬于199培养液中,取上述悬液20μl置于玻片上,室温(24℃)干燥(1～5天内使用)或冰冻(-80℃)保存供以后使用。制备伯氏疟原虫、约氏疟原虫、间日疟原虫及鸡疟原虫子孢子等4种抗原。抗血清的制备:新分离的或钴~(60)照射的伯氏疟原虫、约氏疟原虫子孢子静脉接种小白鼠,或用感染蚊叮咬兔;用伯氏疟原虫的红内期,经静脉注射大白鼠,约氏疟原虫红内期免疫小白鼠,制备抗血清。接种子孢子的动
This article reports the use of serological methods to identify species of sporozoites obtained from mosquitoes in the field. Antigen preparation: Freshly killed or cryopreserved mosquitoes were rapidly centrifuged by discontinuous gradient centrifugation according to the method of Pacheco et al. (1979), and the sporozoites were isolated and suspended in 199 medium at a concentration of 50-150 / μl of sporozoites Take 20 μl of the above suspension and place it on a glass slide and store at room temperature (24 ° C) for 1 to 5 days or freeze (-80 ° C) for later use. Preparation of Plasmodium berghei, Plasmodium yoelii, Plasmodium vivax and Plasmodium sporozoites and other four kinds of antigens. Preparation of antiserum: newly isolated or cobalt ~ (60) irradiated P. berghei and P. yohii sporozoites were inoculated intravenously in mice or bitten rabbits with infected mosquitoes; in the red stage of Plasmodium berghei, Intravenous injection of rats, Plasmodium yoelion red immune mice, preparation of antiserum. Inoculation of sporozoites