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目的:研究喉鳞状细胞癌组织Caveo-lin-1的表达情况及其与HPV感染之间关系,探讨两者对喉鳞癌生物学行为的影响。方法:分别应用免疫组织化学和原位杂交方法检测喉鳞癌组织中Caveolin-1蛋白及HPV感染的情况,统计学分析两者之间的相关性。结果:Caveolin-1蛋白在喉鳞癌和癌旁组织中的表达率分别为71.2%和9.5%,两者表达差异有统计学意义,P<0.05;Caveolin-1蛋白的表达程度与病理类型、TNM分期、转移相关。喉鳞癌组织HPV感染阳性率为55.1%,癌旁组织为21.4%,两者表达差异有统计学意义,P<0.05;HPV感染与患者年龄、病理类型、TNM分期和转移均无关。喉鳞癌Caveolin-1表达与HPV感染之间呈正相关表达(r=0.62,χ2=10.40,P<0.05)。结论:喉鳞癌组织Caveolin-1表达明显增高并与肿瘤的浸润和转移行为有关,Caveolin-1为判断喉鳞癌生物学的指标之一;HPV感染是喉鳞癌发生的高危因素,并且Caveolin-1与HPV两者之间存在协同作用。
Objective: To investigate the expression of Caveo-lin-1 in laryngeal squamous cell carcinoma (LSCC) and its relationship with HPV infection, and to explore the influence of both on the biological behavior of LSCC. Methods: The expressions of Caveolin-1 protein and HPV in laryngeal squamous cell carcinoma tissues were detected by immunohistochemistry and in situ hybridization respectively. The correlation between them was statistically analyzed. Results: The expression rates of Caveolin-1 protein in laryngeal squamous cell carcinoma and paracancerous tissues were 71.2% and 9.5% respectively, the difference was statistically significant (P <0.05). The expression of Caveolin-1 protein correlated with pathological type, TNM staging, transfer related. The positive rate of HPV infection in laryngeal squamous cell carcinoma was 55.1% and that in adjacent tissues was 21.4%, the difference was statistically significant (P <0.05). HPV infection was not associated with age, pathological type, TNM stage and metastasis. The expression of Caveolin-1 in laryngeal squamous cell carcinoma was positively correlated with HPV infection (r = 0.62, χ2 = 10.40, P <0.05). Conclusions: The expression of Caveolin-1 in laryngeal squamous cell carcinoma was significantly increased and correlated with the invasion and metastasis of the tumor. Caveolin-1 was one of the indicators to determine the biology of laryngeal squamous cell carcinoma. HPV infection was a risk factor for laryngeal squamous cell carcinoma, There is synergy between -1 and HPV.