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目的 探讨人巨细胞病毒 (HCMV)对人巨核系祖细胞增殖的影响。方法 收集 2 0份脐血标本 ,采用巨核系祖细胞体外半固体培养技术 ,观察HCMVAD16 9株对巨核细胞集落形成单位 (CFU MK)生长的影响 ,分别用原位聚合酶链反应 (IS PCR)和RT PCR检测集落细胞中的HCMVDNA与即刻早期抗原 (IEA)mRNA。结果 HCMVAD16 9株在体外能明显抑制CFU MK生长 ,抑制程度与病毒感染滴度呈剂量依赖关系 ;经IS PCR检测发现病毒感染组CFU MK细胞中有HCMVDNA存在 ;RT PCR检测发现病毒感染组CFU MK细胞中有IEAmRNA的表达。结论 HCMVAD16 9株可直接感染巨核系祖细胞 ,并抑制其增殖与分化 ;HCMV对巨核系祖细胞的直接抑制作用可能是该病毒感染后引起血小板减少的原因之一
Objective To investigate the effects of human cytomegalovirus (HCMV) on the proliferation of human megakaryocyte progenitor cells. Methods 20 samples of umbilical cord blood were collected and the effects of HCMVAD16 on the proliferation of megakaryocyte colony forming units (CFU MK) were observed by semi-solid culture of megakaryocyte progenitor cells in vitro. The expression of HCMVAD16 was detected by IS PCR, HCMVDNA and immediate early antigen (IEA) mRNA in colony cells were detected by RT PCR. Results HCMVAD16 strain 9 could significantly inhibit the growth of CFU MK in vitro in a dose-dependent manner. The expression of HCMV DNA in CFU MK cells was detected by IS PCR. RT-PCR showed that CFU MK The cells have IEA mRNA expression. Conclusion 9 HCMVAD16 strains can directly infect megakaryocyte progenitor cells and inhibit their proliferation and differentiation. The direct inhibition of HCMV on megakaryocyte progenitor cells may be one of the causes of thrombocytopenia after HCMV16 infection