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丝裂原活化蛋白(MAP)激酶广泛地存在于酵母和哺乳动物的真核细胞内,它们是控制细胞增殖与分化的激酶连锁体,受膜表面受体信号激活调控。为了弄清疟原虫在各个发育时期中MAP激酶的作用,作者应用分子基因技术,从恶性疟原虫(Pf)cDNA及基因组DNA文库中克隆出一株与MAP激酶相似的基因。 按Trager ＆ Jensen法培养Pf 3D_7克隆(为NP54分离株的克隆);加入5％山梨醇
Mitogen-activated protein (MAP) kinases are widely found in yeast and mammalian eukaryotic cells. They are kinases that control cell proliferation and differentiation and are regulated by the activation of membrane surface receptors. To understand the role of MAP kinase in Plasmodium at various developmental stages, the authors used molecular gene technology to clone a gene similar to MAP kinase from P. falciparum (Pf) cDNA and genomic DNA libraries. Pf 3D_7 clone (clone of NP54 isolate) was cultured according to Trager & Jensen method; 5% sorbitol