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目的:观察理筋正骨手法对膝骨关节炎(KOA)模型兔的治疗作用及对其软骨细胞凋亡率、白细胞介素(IL)-1β和一氧化氮(NO)浓度的影响.方法:按照随机数字表法将30只新西兰大白兔进行分组,正常组9只,其余21只为造模组.造模组采用改良Hulth法建立KOA模型.8周时处死3只造模兔以验证模型是否成功.其余18只兔随机分为模型组和干预组,每组9只.正常组与模型组均常规饲养,不施加任何干预手段;干预组予以理筋正骨手法干预,每次10 min,隔日1次,共治疗20次.使用Lequesne MG膝关节功能评级评估各组兔的行为学差异,用Pelletier评分评估兔的大体变化,用Mankin评分评估膝关节软骨的病理情况,用酶联免疫吸附法和硝酸还原酶法分别测定各组血清和关节液中IL-1β和NO浓度,用原位末端标记法测定各组软骨细胞凋亡情况.结果:与正常组相比,模型组的Lequesne MG、Pelletier和Mankin评分及IL-1β、NO浓度增加(P<0.05),间接提示造模成功.与模型组相比,干预组的Lequesne MG、Pelletier和Mankin评分,IL-1β和NO浓度,以及软骨细胞凋亡率下降,差异具有统计学意义(P<0.05).结论:理筋正骨手法可降低IL-1β及NO水平,减轻软骨细胞凋亡率,延缓关节软骨退行性变,对KOA具有良好的治疗作用.“,”Objective: To observe the therapeutic effect of sinew-regulating bone-setting manipulations for knee osteoarthritis (KOA) model rabbits and its impacts on the chondrocyte apoptosis rate and the levels of interleukin (IL)-1β and nitric oxide (NO). Methods: According to the random number table method, 30 New Zealand white rabbits were divided into a normal group (n=9) and a modeling group (n=21). Rabbits in the modeling group were used to establish KOA models with the modified Hulth method. At the 8th week, three rabbits were sacrificed to verify the model and the remaining 18 rabbits were randomly divided into a model group (n=9) and an intervention group (n=9). Rabbits in the normal group and model group were bred routinely without any intervention. Rabbits in the intervention group were treated with the sinew-regulating bone-setting manipulations, 10 min/time, once every other day for a total of 20 times. The Lequesne MG knee function rating was used to evaluate the behavioral differences of the rabbits in each group. The Pelletier score was used to evaluate the general changes of the rabbits. The Mankin score was used to evaluate the pathology of knee cartilages. The enzyme-linked immunosorbent assay and nitrate reductase methods were used to determine the levels of IL-1β and NO in serum and synovial fluid of each group, respectively. In situ terminal deoxynucleotidyl transferase-mediated nick and labeling method was used to determine the apoptosis of chondrocytes in each group. Results: Compared with the normal group, the scores of Lequesne MG, Pelletier and Mankin, and the levels of IL-1β and NO in the model group were increased (P<0.05), which indirectly indicated the success of the model. Compared with the model group, the scores of Lequesne MG, Pelletier and Mankin, IL-1β and NO levels, and chondrocyte apoptosis rate of the intervention group were decreased, and the differences were statistically significant (P<0.05). Conclusion: The sinew-regulating bone-setting manipulations can reduce the levels of IL-1β, NO, and chondrocyte apoptosis rate, and delay the articular cartilage degeneration, therefore, having a good therapeutic effect on KOA.