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为了研究α-酮戊二酸(α-ketoglutarate,AKG)对杂交鲟Acipenser schrenckii♀×Acipenser baeri♂肠道形态、消化酶活力和抗氧化能力的影响,在22℃条件下用添加AKG的饲料饲喂体质量为(7.65±0.04)g的杂交鲟,试验分为4组,每组设5个平行,每个平行放25尾鱼,试验饲料分为大豆浓缩蛋白源、鱼粉与大豆浓缩蛋白复合蛋白源两种,每种蛋白源饲料均添加不同浓度(0和1%)的α-酮戊二酸,用4种试验饲料分别饲喂4组体质量为(7.65±0.04)g的杂交鲟鱼8周。结果表明:AKG能显著提高杂交鲟后肠绒毛高度(P<0.05),不同蛋白源对肠道形态均无显著影响(P>0.05),蛋白源和AKG对肠道形态无交互作用(P>0.05);AKG能显著提高杂交鲟蛋白酶活性(P<0.05),但对脂肪酶和淀粉酶无显著影响(P>0.05),大豆浓缩蛋白源能显著提高蛋白酶和淀粉酶活性(P<0.05),复合蛋白源对肠道消化酶活性无显著影响(P>0.05),蛋白源和AKG对脂肪酶和淀粉酶活力有交互作用(P<0.05);AKG能显著提高肠道中SOD活性并显著降低MDA含量(P<0.05),但对GSH和CAT含量均无显著影响(P>0.05),不同蛋白源对肠道抗氧化能力均无显著影响(P>0.05),蛋白源和AKG对MDA含量有交互作用(P<0.05),对其他抗氧化指标均无交互作用(P>0.05)。研究表明,饲料中添加AKG可以促进杂交鲟肠道发育,提高肠道消化酶活性和抗氧化能力。
In order to study the effect of α-ketoglutarate (AKG) on intestinal morphology, digestive enzyme activity and antioxidant capacity of Acipenser schrenckii♀ × Acipenser baeri♂, The experiment was divided into 4 groups, 5 parallel in each group, with 25 fish in parallel. The experimental diets were divided into soy protein concentrate, fish meal and soybean protein concentrate Two kinds of protein sources were used, and α-ketoglutarate with different concentrations (0 and 1%) was added into each protein source feed. Four groups of test diets were fed with four groups of hybrid sturgeons (7.65 ± 0.04) g Fish 8 weeks. The results showed that: AKG could significantly increase the villus height of hybrid sturgeon (P <0.05), and different protein sources had no significant effect on intestinal morphology (P> 0.05). There was no interaction between protein source and AKG on intestinal morphology (P> 0.05). AKG could significantly increase the protease activity of hybrid sturgeon (P <0.05), but had no significant effect on lipase and amylase (P> 0.05). The protein source of soybean protein concentrate could significantly increase the activity of protease and amylase (P <0.05) (P> 0.05). The protein source and AKG interacted with the activity of lipase and amylase (P <0.05). AKG significantly increased the activity of intestinal enzymes and decreased the activity of intestinal enzymes (P <0.05), but had no significant effect on the contents of GSH and CAT (P> 0.05). There were no significant effects of different protein sources on the intestinal antioxidant capacity (P> 0.05) There was interaction (P <0.05), but no interaction with other antioxidant indexes (P> 0.05). Studies have shown that the addition of AKG feed can promote intestinal development of hybrid sturgeon, improve intestinal digestive enzyme activity and antioxidant capacity.