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背景:目前,深静脉血栓的分子病因学机制及其形成的核心调控网络仍未完全阐明,对于深静脉血栓的早期诊断预测也无理想的方法。目的:研究组织蛋白酶L/G与创伤性深静脉血栓的预测。方法:采用蚊式钳夹闭50只SD大鼠双侧股静脉的3个不同部位3s随后予以模具制动制备大鼠创伤性深静脉血栓模型,根据股静脉血栓形成的不同阶段和生物学特征,将模型大鼠分为血栓形成前组、血栓形成组和无血栓形成组,另取10只正常大鼠作为对照组。在相应时间点取大鼠创伤静脉,提取总RNA,经过基因芯片技术筛选差异表达基因,并进一步应用real-time PCR进行验证。结果与结论:基因芯片杂交结果发现组织蛋白酶L/G基因在各组间差异表达明显,其中血栓形成组最高,无血栓形成组和血栓形成前组次之,均明显高于对照组(P<0.05);real-time PCR分析结果与基因芯片杂交分析结果相一致。说明局部静脉血管壁中组织蛋白酶L/G表达水平升高与创伤性深静脉血栓形成有关,可作为深静脉血栓形成早期诊断、预测的候选分子标志。
BACKGROUND: At present, the molecular etiology mechanism of deep venous thrombosis and the formation of its core regulatory network are still not completely elucidated. There is also no ideal method for the early diagnosis of deep venous thrombosis. Objective: To study the prediction of cathepsin L / G and traumatic deep vein thrombosis. Methods: Three different parts of bilateral femoral veins of 50 SD rats were closed by mosquito forceps for 3s, then the model of traumatic deep venous thrombosis was prepared by mold brake. According to the different stages and biological characteristics of femoral vein thrombosis, The model rats were divided into thrombosis group, thrombosis group and non-thrombosis group, and another 10 normal rats were taken as the control group. At the corresponding time point, the wounds of rats were taken out and the total RNA was extracted. The differentially expressed genes were screened by gene chip technology and further verified by real-time PCR. RESULTS AND CONCLUSION: The results of gene chip hybridization showed that the expression of cathepsin L / G gene was significantly different among the three groups. The thrombosis group, thrombosis group and thrombosis group were significantly higher than the control group (P < 0.05). The result of real-time PCR was consistent with that of gene chip hybridization. These findings suggest that the elevated expression of cathepsin L / G in the local venous wall may be related to traumatic deep vein thrombosis, which may serve as a candidate molecular marker for the early diagnosis and prediction of deep venous thrombosis.