Effects of 17β-estradiol on the expressions of CTGF and PAIP-1 in MG-63 cells

• 作 者: 翟木绪
• 机 构: Instit Metab & Endocrinol 2nd Xiangya Hosp Centr South Univ,Changsha 410011,
• 出 处: China Medical Abstracts(Internal Medicine)
• 发表日期: 2006年04期
• 关键词: estradiol interacting alkaline connective stained collagen mineralization staini

Objective To observe the expressions of connective tissue growth factor (CTGF) and polyadenylate-binding protein interacting protein-1 (PAIP-1) mRNA during MG-63 cell proliferation and differentiation, and to investigate the effect of 17β-estradiol (E2) on the expressions of CTGF and PAIP-1 mRNA. Methods The expressions of typeⅠcollagen, alkaline phosphatase (ALP) and osteocalcin mRNA were determined by semiquantitative RT-PCR. Cultured cells were stained with

Objective To observe the expressions of connective tissue growth factor (CTGF) and polyadenylate-binding protein interacting protein-1 (PAIP-1) mRNA during MG-63 cell proliferation and differentiation, and to investigate the effect of 17β-estradiol The expressions of CTGF and PAIP-1 mRNA. Methods The expressions of typeⅠcollagen, alkaline phosphatase (ALP) and osteocalcin mRNA were determined by semiquantitative RT-PCR. Cultured cells were stained with