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目的 :建立反相HPLC法定量分析尿液中的他扎司特体内活性代谢物 ,研究其尿药动力学特征。方法 :8名健康受试者口服单剂量 75mg他扎司特胶囊后 ,按时间段收集尿液 ,反相HPLC法测定尿中活性代谢物浓度 ,考察 16h内尿药排泄变化。尿药排泄数据处理采用亏量法。结果 :主要药动学参数 :消除半衰期 (t1/ 2 )为 (1.8± 0 .6 )h ,累积尿药排泄量 (Xu∞)为(2 8.8± 7.7)mg ,累积排泄率 (P % )为 (48.0± 10 .6 ) %。结论 :本方法适用于他扎司特的药动学研究及临床尿药浓度监测
Objective: To establish a reversed-phase HPLC method for the quantitative analysis of active metabolites of tazetactone in urine and to study its urodynamic characteristics. Methods: Eight healthy volunteers received a single oral dose of 75 mg tazobactam. Urine was collected by time and the concentrations of active metabolites in the urine were determined by reverse phase HPLC. Urine excretion was determined within 16 hours. Urine excretion data processing using the deficit method. RESULTS: The main pharmacokinetic parameters were as follows: elimination half-life (t1 ± 2) was (1.8 ± 0.6) h, cumulative excretion rate (8.8 ± 7.7) mg and cumulative excretion rate (48.0 ± 10 .6)%. Conclusion: This method is suitable for the study of tazobactam pharmacokinetics and clinical urinary drug concentration monitoring