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代料袋栽香菇一般都是采用木屑菌种接种,工序较繁,成本高,且容易污染。为此,我们作了菌种的改形及接种方法的改进试验,收到了较好效果,现介绍如下:(一)菌种制作①采用阔叶树或竹,锯成3～4cm长,用刀劈成6mm 厚的木、竹片,并将木、竹片的一头两边削去2/3厚使成刀口状,然后劈成正方形的颗粒状木、竹钉待用。②将木、竹钉浸在1%糖、1%CaCO_3或石膏粉水溶液中12小时,捞起滤去多余的水溶掖,再加少量的麸皮、木屑拌匀,使每粒木、竹钉都沾上麸皮、木屑原料。③将拌好的木钉装入广口瓶,装量为瓶高的2/3,约150粒,上面盖一层拌好的剩余碎料约5mm,压平固定,高压灭菌1.5小时。④待温度降至25℃时接种,培养时间要比木屑种长7天左右,长好的菌种菌丝洁白,生长旺盛。
Substitute bag mushrooms are generally inoculated with sawdust species, process more complicated, high cost, and easy to pollute. To this end, we made strains of the modified and inoculation methods to improve the test, received better results, are described below: (A) strain production ① broad-leaved trees or bamboo, sawing into 3 ~ 4cm long, with a knife split Into a 6mm thick wood, bamboo, and wood, bamboo on both sides of a cut 2/3-thick knife into the mouth, and then split into square granular wood, bamboo nail to be used. ② The wood, bamboo spike immersed in 1% sugar, 1% CaCO_3 or gypsum powder aqueous solution for 12 hours, picked up the excess water to remove the tuck, plus a small amount of bran, mix well, so that each piece of wood, bamboo nail All stained with bran, wood raw materials. ③ The spiked wooden spike into the jar, the amount of bottle height of 2/3, about 150, covered with a layer of the remaining pieces of the remaining good about 5mm, flattened, autoclaved 1.5 hours. ④ until the temperature dropped to 25 ℃ inoculation, culture time than sawdust long about 7 days, grow well mycelium white, vigorous growth.