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目的 探讨肝树突状细胞（ｈｅｐａｔｉｃ ｄｅｎｄｒｉｔｉｃ ｃｅｌｌ，ＨＤＣ）的生物主疫功能及在肝移植耐受中的作用。方法 分别从肝脏和骨髓分离、培养ＤＣ，以免疫组织化学及流式细胞术检测两种ＤＣ生物特性及免疫活性的差异。结呆 培养６～８ｄ，骨髓 ＤＣ（ＢＭ－ＤＣ）有大量细胞从集落中释放，并呈成熟状态，细胞表面组织相容性抗原 ＭＨＣ ｃｌａｓｓ、ＭＨＣ ｃｌａｓｓ Ⅱ及共刺激分子ＣＤ８０（Ｂ７－１）、ＣＤ８６（Ｂ７－２）都有较高水平表述，混合淋巴细胞反应（ＭＬＲ）中表现出有强烈刺激Ｔ细胞增生的能力；而同时期的ＨＤＣ具有非成熟ＤＣ的形态及功能特征，表面分子表达很低，无刺激Ｔ细胞增生的能力。结论 实验结果提示ＨＤＣ具有ＢＭ－ＤＣ不同的生物特性可能是产生肝脏移植受特性的一个重要原因。
Objective To investigate the biological function of liver dendritic cells (HDC) and its role in liver transplantation tolerance. Methods DCs were isolated and cultured from liver and bone marrow respectively. Immunohistochemistry and flow cytometry were used to detect the difference of biological characteristics and immunological activities between the two DCs. After cultured for 6-8 days, many cells in bone marrow DC (BM-DC) were released from the colonies and matured. The histocompatibility antigens MHC class, MHC class II and costimulatory molecule CD80 (B7-1) , CD86 (B7-2) all showed higher level expression, and mixed lymphocyte reaction (MLR) showed a strong ability to stimulate T cell proliferation; while the same period of HDC with morphological and functional characteristics of immature DC, surface molecules Low expression, no stimulation of T cell proliferation. Conclusion The experimental results suggest that different biological characteristics of HDC with BM-DC may be an important reason for the characteristics of liver transplantation.