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目的:观察活性氧(ROS)是否参与大剂量氯胺酮致原代培养皮层神经元凋亡。方法:单纯培养基(C组)、1mmol/L氯胺酮(K组)、1mmol/L氯胺酮复合2.5μmol/L超氧化物歧化酶类似物M40403(M组)分别作用于体外原代培养第6天的神经元24h,检测神经元ROS生成、凋亡细胞百分比及促凋亡蛋白Bax表达。结果:与C组相比,K组神经元ROS生成、凋亡细胞百分比、Bax表达分别是其1.7、4.2及2.0倍(P<0.05);与C组相比,M组神经元ROS生成、凋亡细胞百分比、Bax表达差异无统计学意义。结论:ROS介导大剂量氯胺酮致原代培养皮层神经元凋亡。
AIM: To observe whether reactive oxygen species (ROS) is involved in the apoptosis of primary cultured cortical neurons induced by high dose ketamine. Methods: Cultured in vitro (group C), 1 mmol / L ketamine (K group), 1 mmol / L ketamine combined with 2.5 μmol / L superoxide dismutase M40403 (M group) Of neurons 24h detection of ROS generation neurons, the percentage of apoptotic cells and pro-apoptotic protein Bax expression. Results: Compared with group C, ROS production, percentage of apoptotic cells and expression of Bax in K group were 1.7, 4.2 and 2.0 times higher than those in group C (P <0.05). Compared with group C, ROS production, There was no significant difference in the percentage of apoptotic cells and Bax expression. Conclusion: ROS induces apoptosis of primary cultured cortical neurons induced by high dose ketamine.