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目的:探讨MTDH基因对乳腺癌MDA-MB-453细胞增殖、黏附和迁移能力的影响。方法:采用RNA干扰技术,下调乳腺癌MDA-MB-453细胞MTDH基因的表达.RT-PCR和免疫细胞化学技术检测MTDH下调效果.通过细胞增殖实验、黏附实验和迁移实验分别检测MTDH基因表达下调后细胞增殖、黏附和迁移能力的变化。结果:MTDH-siRNA的转染效率达到90%,转染48 11后实验组细胞MTDH的mRNA和蛋白质表达较对照组分别降低了45.8<%和47.5%MTDH表达下调后,细胞增殖受到明显抑制,48 h和72 h抑制率分别为41.5%和49.0%;细胞黏附力下降,30min和60 imn黏附率较对照组分别降低42.0%和49.7%;细胞迁移能力显著降低,迁移率下降333%。结论:下调MTDH基因表达可明显抑制乳腺癌MDA-MB-453细胞的增殖、黏附和迁移能力,提示其在乳腺癌细胞的恶性生物学行为中发挥重要作用。
Objective: To investigate the effect of MTDH gene on the proliferation, adhesion and migration of breast cancer MDA-MB-453 cells. Methods: The MTDH gene expression in breast cancer MDA-MB-453 cells was down-regulated by RNA interference technique. The down-regulation effect of MTDH was detected by RT-PCR and immunocytochemistry. MTDH gene expression was detected by cell proliferation assay, adhesion assay and migration assay Post cell proliferation, adhesion and migration ability changes. Results: The transfection efficiency of MTDH-siRNA reached 90%. The MTDH mRNA and protein expression in the experimental group decreased by 45.8% and 47.5% respectively after 48 11 transfection, and the cell proliferation was significantly inhibited after MTDH-siRNA transfection. 48 h and 72 h inhibition rates were 41.5% and 49.0%, respectively; cell adhesion decreased, the adhesion rate of 30min and 60 imn decreased by 42.0% and 49.7% respectively compared with that of the control group; the migration ability decreased significantly and the mobility decreased by 333%. CONCLUSION: Down-regulation of MTDH gene expression can significantly inhibit the proliferation, adhesion and migration of breast cancer MDA-MB-453 cells, indicating that it plays an important role in the malignant biological behavior of breast cancer cells.