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以嫩丰20为试验材料,在温室和盆栽条件下,以清水为对照,用15%的PEG-6000模拟干旱条件,设置4个浓度(0,20,40和60 mg·L~(-1))的DCPTA溶液处理进行叶面喷施,考察干旱胁迫下其对大豆幼苗叶片光合特性、保护酶以及叶绿素荧光特性的影响。结果表明:DCPTA处理提高了苗期大豆叶片光合速率、蒸腾速率、气孔导度和叶绿素含量,降低了胞间CO_2浓度;提高了最大荧光量(Fm)、PSⅡ的最大光能转换速率(Fv/Fm)和光化学淬灭系数(q P);降低了非光化学淬灭系数(q N);增加了保护酶活性;并促进了脯氨酸的积累。过氧化氢酶(CAT)活性和脯氨酸含量以60 mg·L~(-1)为最优处理,其余指标均以40 mg·L~(-1)处理为最优。
Nenfeng 20 was used as test material to simulate drought conditions with 15% PEG-6000 under the condition of greenhouse and potted plants. Four concentrations (0, 20, 40 and 60 mg · L -1 )) DCPTA solution was applied to foliar spray to investigate the effects of drought stress on photosynthetic characteristics, protective enzymes and chlorophyll fluorescence of soybean seedling leaves. The results showed that DCPTA treatment increased photosynthetic rate, transpiration rate, stomatal conductance and chlorophyll content of soybean seedling at seedling stage, decreased intercellular CO_2 concentration, enhanced maximum fluorescence (Fm), maximum photochemical conversion rate (Fv / Fm) and photochemical quenching coefficient (q P); reduced non-photochemical quenching coefficient (q N); increased protective enzyme activity; and promoted proline accumulation. Catalase (CAT) activity and proline content were optimally treated with 60 mg · L -1, and the other indexes were all optimized by 40 mg · L -1 treatment.