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一、马铃薯组织培养 马铃薯为无性繁殖作物,滤过性病毒经营养器官代代相传。台湾马铃薯脱毒种苗有两条途径: 1.分生组织法:将已打破休眠的块茎用1000倍万力及链霉素溶液浸种三小时后,在消毒的(石至)石中催芽,并定期喷施杀菌剂,以获得半无菌状态的外植体。而获得无病毒苗最安全的方法是培养不带叶原基的生长点(约100μ长),其次是含有一叶原基的茎顶圆锥体(200 μ长)。由培养成活率和去除病毒的效果结合考虑,则以培养200μ长的茎顶获得无毒苗的效率最高。
First, the potato tissue culture Potato is a vegetative propagation of the virus by the vegetative organs from generation to generation. Taiwan potato virus-free seedlings in two ways: 1. Meristem tissue method: the dormant tubers have been broken 1000 times the strength and streptomycin solution soaking three hours after the disinfection (stone to) stone in the germination, And regularly spray fungicides to obtain semi-sterile explants. The safest way to obtain vaccine-free seedlings is to grow growth points without leaf primordia (about 100 μL) followed by stem apices (200 μL) containing a leaf primordium. Considering the effects of culturing the survival rate and removing the virus, it is most efficient to obtain a non-toxic vaccine by cultivating a 200-μ-long stem top.