论文部分内容阅读
目的:研究腔隙性梗死(腔梗)与皮质、皮质下梗死(皮梗)患者的载脂蛋白E(ApoE)基因多态性分布的差异及与多种血管危险因素相关性的差异。方法:设腔梗组(81例)和皮梗组(87例),以调查表方式调查患者一般状况及血管危险因素;晨起空腹采血分离血清,测血糖、血脂,并以聚合酶链反应-限制性片段长度多态性(PCR-RFLP)检测法测定患者ApoE基因型。结果:①2组基因分布:腔梗组ε3/4+ε4/4型频率(25.9%)明显高于皮梗组(11.5%);ε4频率(17.9%)高于皮梗组(9.8%),P均<0.05。ε3/4及ε4/4的携带者发生腔梗危险度高于皮梗,OR=2.77,95%CI1.21~6.38,P<0.05。②腔梗组高血压患者多于皮梗组,高血压患者患腔梗,危险度大于皮梗,OR=2.02,95%CI1.07~3.80,P<0.05,。腔梗组血浆总胆固醇水平高于皮梗组,P<0.05。结论:ε3/4和ε4/4携带者发生腔梗的危险度大于皮梗,这可能和ε4增高TC水平,加速脑小动脉粥样硬化进程有关。
OBJECTIVE: To study the differences of polymorphism of apolipoprotein E (ApoE) gene between lacunar infarction (CA) and cortical and subcortical infarcts (PDT) and the correlation with the risk of various vascular diseases. Methods: A total of 81 cases of CAI group and 87 cases of TBI group were enrolled in this study. The general condition and vascular risk factors were investigated by questionnaire. Serum fasting blood samples were taken from early morning to test blood glucose and blood lipids. Polymerase chain reaction ApoE genotypes were determined by restriction fragment length polymorphism (PCR-RFLP). Results: (1) The distribution of gene in group 2: The frequency of ε3 / 4 + ε4 / 4 in CA group was significantly higher than that in TB group (11.5%); ε4 frequency (17.9%) was higher than TB group (9.8% P <0.05. The carriers with ε3 / 4 and ε4 / 4 had a higher risk of lumen infarction than those with PE, OR = 2.77, 95% CI1.21 ~ 6.38, P <0.05. ② There were more patients with hypertension in the infarction group than those in the dermatophyte group. The patients with hypertension had lumen infarction and the risk was greater than that of the dermatophyte (OR = 2.02, 95% CI 1.07 ~ 3.80, P <0.05). The total plasma cholesterol level in the lumen group was higher than that in the pee group, P <0.05. CONCLUSIONS: The risk of stenosing the infarct in ε3 / 4 and ε4 / 4 carriers is greater than that in the susceptible dermatitis, which may be related to increasing the level of TC and accelerating the process of cerebrovascular atherosclerosis.