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目的探讨桃花多酚对慢性应激诱导的抑郁小鼠干预作用及其对脑海马五羟色胺、去甲肾上腺素水平影响。方法将50只昆明小鼠根据体重和1%蔗糖水偏嗜度随机分成5组,即模型对照组,氟西汀组,桃花多酚低、中、高剂量组(20、40、80 mg/kg);利用慢性轻度不可预见性刺激制造抑郁动物模型,观察模型小鼠行为改变及海马组织五羟色胺、去甲肾上腺素含量。结果模型组,氟西汀组,低、中、高桃花酚组小鼠逃避失败次数分别为(18.60±2.72)、(3.20±1.23)、(15.10±2.23)、(7.00±2.67)、(4.70±2.06)次;小鼠悬尾不动时间分别为(13.20±2.90)、(3.90±1.20)、(9.00±2.31)、(5.90±2.51)、(4.20±1.40)s;与模型组比较,桃花多酚各剂量组小鼠逃避失败次数降低且小鼠悬尾不动时间缩短,中、高剂量组均优于低剂量组(P<0.01);桃花多酚各剂量均能升高脑海马五羟色胺和去甲肾上腺素含量(P<0.01),与氟西汀具有相似抗郁效果。结论桃花多酚能明显增加脑海马五羟色胺和去甲肾上腺素含量,其抗抑郁作用机制可能与之有关。
Objective To investigate the effects of peach polyphenols on chronic stress-induced depression in mice and its effect on hippocampal serotonin and norepinephrine levels. Methods Fifty Kunming mice were randomly divided into five groups according to body weight and 1% sucrose water bias, namely model control group, fluoxetine group, peach polyphenol low, medium and high dose groups (20, 40, 80 mg / kg). Chronic mild unpredictable stimulation was used to make animal models of depression, and behavioral changes and serotonin and norepinephrine contents in hippocampus were observed. Results The number of escape failures in model, fluoxetine, low, middle and high peach phenol groups were (18.60 ± 2.72), (3.20 ± 1.23), (15.10 ± 2.23), (7.00 ± 2.67), (4.70 ± 2.06). The immobility time of the mice was (13.20 ± 2.90), (3.90 ± 1.20), (9.00 ± 2.31), (5.90 ± 2.51) and (4.20 ± 1.40) s respectively. Compared with the model group, Peach polyphenols in each dose group decreased the number of escape failure mice tail suspension time shortened, medium and high dose group were better than the low dose group (P <0.01); peach polyphenols can increase the dose of the brain hippocampus Serotonin and norepinephrine (P <0.01), and fluoxetine has similar anti-depressive effect. Conclusion Peach polyphenols can significantly increase the content of serotonin and norepinephrine in hippocampus, and its antidepressant mechanism may be related to it.