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应用反相高效液相色谱法,采用国产液相色谱填料YWG-C18H37,甲醇-水为流动相,分离定量哈士蟆卵油中脂溶性维生素D、E,以及β-胡萝卜素,结果获得满意的分离定量效果,为哈士蟆卵油的应用提供了依据。
The results obtained were satisfactory by RP-HPLC with domestic liquid chromatography packing YWG-C18H37 and methanol-water as mobile phase, and the quantification of fat-soluble vitamin D, E and β-carotene in Asparagus hogmanii oil The quantitative analysis results provide a basis for the application of toad oil.