论文部分内容阅读
目的：探讨碱性成纤维细胞生长因子(ｂＦＧ）与喉癌生长、浸润、转移的关系及其可能的作用机制．方法：用原位杂交的方法观察ｂＦＧＦｍＲＮＡ在正常喉粘膜上皮（Ｎ）、慢性非特异性炎症（ＩＦ）、不典型增生（ＤＹＳ）及鳞癌（ＳＣＣ）中的表达．结果：在Ｎ，ＩＦ．ＤＹＳ及ＳＣＣ的间质中均检测到ｂＦＧＦｍＲＮＡ的表达．上皮细胞未见阳性表达．在Ｎ及ＩＦ，阳性反应部位位于血管内皮细胞、成纤维细胞；ＩＦ的阳性反应略有提高，此外部分炎细胞也见阳性反应；ＤＹＳ及ＳＣＣ中的阳性反应程度较Ｎ及ＩＦ有所增强，尤其是新生血管内皮细胞的阳性率及反应程度明显提高．在ＳＣＣ中，分化差的ｂＦＧＦ表达较分化好的ＳＣＣ阳性反应程度强．结论：ｂＦＧＦ不仅可以促进喉癌新生血管及间质的形成．而且可能参与肿瘤细胞生长．在喉癌中，ｂＦＧＦ以旁分泌生长方式影响肿瘤的生长．
Objective: To investigate the relationship between basic fibroblast growth factor (bFG) and the growth, invasion and metastasis of laryngeal carcinoma and its possible mechanism. Methods: The expression of bFGF mRNA in normal laryngeal mucosal epithelium (N), chronic non-specific inflammation (IF), dysplasia (DYS) and squamous cell carcinoma (SCC) was observed by in situ hybridization. Results: At N, IF. The expression of bFGF mRNA was detected in interstitial of DYS and SCC. No positive expression of epithelial cells. In N and IF, positive reaction site located in vascular endothelial cells, fibroblasts; IF positive reaction slightly increased, in addition some inflammatory cells also see the positive reaction; DYS and SCC positive reaction than N and IF increased, In particular, the positive rate of neovascular endothelial cells and the reaction rate was significantly improved. In SCC, poorly differentiated bFGF expression was more positive than well differentiated SCC. Conclusion: bFGF can not only promote angiogenesis and interstitial formation of laryngeal carcinoma. And may participate in tumor cell growth. In laryngeal cancer, bFGF affects the growth of the tumor in a paracrine manner.