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无融合生殖型平邑甜茶（ＭａｌｕｓｈｕｐｅｈｅｎｓｉｓＲｅｈｄ．）实生苗木在形态上整齐一致，染色体倍性不一并具混倍现象，这就造成其杂交后代中有性杂种难以早期准确鉴定。ＲＡＰＤ技术能简便迅速地解决这一问题，且比形态学和细胞学方法准确度高。在平邑甜茶×扎矮７６（Ｍ．ｂａｃ－ｃａｔａＢｏｒｋｈ）的杂交后代中绝大部分属于母本基因型，杂种比率仅占８％左右。其中一半杂种为皱叶型杂种，易于通过皱叶性状鉴别出来，另一半属普通型杂种，拟通过ＲＡＰＤ技术鉴别。实验中共筛选了８０个随机引物，获得一对共显性标记ＯＰＢ０７－５００与ＯＰＢ０７－４５０和一个纯合显性标记ＯＰＨ０４－８００，用这两个标记中的任意一个（对）标记即可鉴定出全部的有性杂种。采用ＤＮＡ混样分析法，将待测株系的ＤＮＡ样品分组混合后进行ＲＡＰＤ－ＰＣＲ扩增，对含有父本特异标记的小组再分株进行鉴定，降低了实验成本。由于需通过ＲＡＰＤ标记鉴定的杂种仅占４％左右，故在分组中将４～６株待测后代分为一组，这样降低了实验次数，使实验费用减少了６０％左右。
Non-fusion reproductive Malpighia spp. (MalushupehensisRehd.) Seedlings in the shape of neat, chromosomal ploidy with a mixed phenomenon, which led to hybrid sexual offspring in early hybrids difficult to accurately identify. The RAPD technique can solve this problem easily and quickly, and is more accurate than the morphological and cytological methods. Most of the offspring of M.bac-cataBorkh hybrids belonged to the female parent genotype, and the proportion of hybrids accounted for only about 8%. Half of the hybrids are polygamous, easily identified by foliar leaf traits and the other half are common type hybrids that are to be identified by RAPD. A total of 80 random primers were screened in the experiment to obtain a pair of co-dominant markers OPB07-500 and OPB07-450 and a homozygous dominant marker OPH04-800, which can be identified by using any of these two markers Out of all the sex hybrids. Using DNA mixed analysis method, the DNA samples of the tested strains were mixed and then amplified by RAPD-PCR, and then the group containing the male-specific marker was re-segmented to be identified, reducing the experimental cost. Because only about 4% of the hybrids need to be identified by RAPD markers, 4 to 6 offspring to be tested are grouped into groups, which reduces the number of experiments and reduces the experimental cost by about 60%.