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目的研究锰对神经细胞内钙稳态的影响,重点观察神经元细胞钙离子浓度、Na+-K+-ATPase和Ca2+-ATPase活性的改变。方法选用原代培养神经元为模型,待细胞生长至最佳状态时,予以分组处理:锰处理组为含不同浓度氯化锰(0,25,100,400μmol/L)的培养液,培养神经细胞12h后,通过倒置相差显微镜观察细胞形态的改变,并检测神经元细胞内钙离子浓度、Na+-K+-ATPase和Ca2+-ATPase活性的变化。结果随着染Mn剂量的加大,神经元形态出现异常,细胞内钙离子浓度逐渐升高;细胞Na+-K+-ATPase和Ca2+-ATPase活性也逐渐下降。结论锰通过影响与维持钙稳态相关的酶(Na+-K+-ATPase和Ca2+-ATPase)活性,干扰神经元细胞内钙稳态,进而造成神经元细胞损伤。
Objective To study the effect of manganese on the calcium homeostasis in neurons and to observe the change of calcium ion concentration, Na + -K + -ATPase and Ca2 + -ATPase in neurons. Methods Primary cultured neurons were selected as models. When the cells grew to their optimal state, the cells were treated with different concentrations of manganese chloride (0, 25, 100, 400 μmol / L) for 12 h , The changes of cell morphology were observed by inverted phase contrast microscope, and the changes of intracellular calcium concentration, Na + -K + -ATPase and Ca2 + -ATPase activity were detected. Results As the dose of Mn was increased, the morphology of neurons became abnormal and the intracellular calcium concentration gradually increased. The activity of Na + -K + -ATPase and Ca2 + -ATPase also decreased gradually. Conclusion Manganese interfered with the intracellular calcium homeostasis by affecting the activity of enzymes (Na + -K + -ATPase and Ca2 + -ATPase) associated with maintaining calcium homeostasis, leading to neuronal cell injury.