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根据黄萎病菌在培养基上能形成微菌核的特性,寻找适于种子与土壤中黄萎病菌微菌核形成的培养基和相应的检查种籽和土壤带菌的方法。1.试验结果表明,棉籽饼粉酒精洋菜培养基(棉籽饼粉10克、95%酒精17毫升、键霉素100p.p.m.、洋菜7.5克、水1000毫升制成)对病菌形成微菌核的效果比 Nadakavakaren 介绍的培养基显著优越。即使对“退化”病菌,亦能促其迅速恢复形成大量微菌核的能力。2.应用上述棉籽饼粉酒精洋菜培养基对从辽阳、临汾和安阳各地黄萎病株上采收的种子进行分离,可得到肉眼可见的微菌核丛,而且具有典型的轮生分生孢子梗,纯化的病菌接种棉苗也表现典型症状。直接证明了这些棉籽确实带有黄萎病菌。用无病田采收的种子进行分离,无微菌核丛形成。3.用上述培养基进行土壤黄萎病菌的分离,也获得比其他培养基显著优越的结果,每皿可出现4—7个微菌核丛。其他培养基仅出现少量甚至完全不形成微菌核。4.上述种子和土壤黄萎病菌分离和检查方法,可试用于种子和土壤带菌的检查及有关种子和土壤消毒效果等的研究。
According to the characteristics of Verticillium dahliae that can form micro-bacterial nuclei on the medium, the paper aims to find the medium suitable for the formation of micro-nucleus of Verticillium dahliae in seeds and soil and the corresponding method of checking the fungi and soil. 1. The test results show that cottonseed cake powder alcohol foreign culture medium (cottonseed cake powder 10 grams, 95% ethanol 17 ml, the key to the penicillin 100p.pm, Agave 7.5 grams, made of 1000 ml of water) on the bacteria to form micro-bacteria The effect of the nucleus is significantly better than the medium described by Nadakavakaren. Even for “degenerate” pathogens, they are also able to prompt their rapid recovery from the ability to form large numbers of microscopic bacterial nuclei. Separation of seeds harvested from Verticillium dahliae strains from Liaoyang, Linfen and Anyang, using the above-mentioned cottonseed meal alcohol agar medium to obtain microscopic nuclear plexus cluster which can be seen macroscopically, Spore stalk, purified bacteria inoculated cotton seedlings also showed typical symptoms. Directly prove that these cottonseeds do indeed carry Verticillium dahliae. Seeds were harvested with disease free field separation, no formation of microscopic nuclear plexus. 3. With the above medium for the isolation of soil Verticillium dahliae, also obtained significantly superior results than other media, each dish can appear 4-7 bacteria cluster. Other media showed little or no micro-sclerotia at all. 4. Seeds and soil Verticillium dahliae separation and inspection methods can be used to test for seed and soil contamination and the disinfection of seeds and soil research.