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研究了甲烷利用菌Methylomonas sp.GYJ3,Methylomonas sp.S,Methylomonaa sp.Z201,Methylococcus capsulatus IMV3021,Methylosinus trichosporium IMV3011休止细胞催化烯烃环氧化的底物选择性,细胞失活原因以及产物对映体组成.发现不同菌株和底物的环氧化活性不同.甲烷利用菌只能催化短链烯烃环氧化,环烯烃和芳香烯烃无反应.对烯丙基型底物而言,取代基大小和极性影响环氧化活性 丙烯环氧化活性最高,烯丙醇不能环氧化.细胞失活的主要原因是环氧化产物的细胞毒性和反应体系中辅酶NADH损耗.手性气相色谱揭示甲烷利用菌催化烯烃环氧化形成外消旋产物.
The substrate selectivity, the cause of cell deactivation, and the enantiomeric composition of the product were investigated using the resting cell-catalyzed epoxidation of methane using bacteria Methylomonas sp. GYJ3, Methylomonas sp.S, Methylomona sp. Z201, Methylococcus capsulatus IMV3021, Methylosinus trichosporium IMV3011 .It was found that the epoxidation activities of different strains and substrates were different.Methanogenic bacteria could only catalyze the epoxidation of short chain olefins and did not react with cyclic olefins and aromatic olefins.For allyl type substrates, Sexual effects Epoxidation activity The highest epoxidation activity of propylene, allyl alcohol can not epoxidation.The main reason for cell deactivation is the cytotoxicity of epoxidation products and the coenzyme NADH loss in the reaction system Chiral gas chromatography reveals methane utilization Bacteria catalyze the epoxidation of olefins to form racemic products.