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目的了解丙型肝炎病毒(HCV)F抗体在南京市吸毒人群HCV感染者中的分布特点,为丙型肝炎防治及制定慢性化预防策略提供依据。方法利用pEGX-4T-2/HCV-F融合载体表达蛋白HCV-F/GST作为抗原,包被酶联反应板,ELISA间接法检测362例HCV感染者及50例正常人血清HCV-F抗体,结合其人口学特征和疾病特征,统计分析HCV-F抗体的分布情况及与HCV感染的关系。结果 362例感染HCV的吸毒者F抗体阳性率为31.5%。通过比较发现F抗体阳性率在性别、吸毒时间、吸毒方式等因素中分布无统计学差异,而F抗体阳性率与年龄、联合感染可能具有一定关联。结论南京市吸毒人群HCV感染者中存在F抗体,其可能与疾病病程相关。
Objective To investigate the distribution of hepatitis C virus (HCV) F antibodies in HCV infected persons in Nanjing, and to provide evidences for the prevention and treatment of hepatitis C and the development of a preventive strategy for chronic diseases. Methods The HCV-F / GST protein was expressed by pEGX-4T-2 / HCV-F fusion protein and coated with enzyme-linked immunosorbent assay. The HCV-F antibody was detected in 362 HCV-infected and 50 normal individuals by ELISA, Combined with its demographic characteristics and disease characteristics, statistical analysis of HCV-F antibody distribution and the relationship between HCV infection. Results The positive rate of F antibody in 362 HCV infected drug users was 31.5%. By comparison, the positive rate of F antibody in sex, drug use time, drug use and other factors in the distribution was not statistically different, and the positive rate of F antibody and age, co-infection may be related. Conclusion There are F antibodies in HCV infected persons in Nanjing drug users, which may be related to the disease course.