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前文曾报导胞二磷胆碱(Cytidine Diphosphocholine,简称CDP-胆碱)有显著抑制贮存红细胞溶血的作用.为探讨CDP-胆碱的作用是否与嘌呤核苷类相同,也参与了细胞内的能量代谢,本文就红细胞贮存过程中的某些生化指标作了分析.我们将含有CDP-胆碱、并以酸性柠檬酸葡萄糖(简称ACD)抗凝的正常人血(CDP-胆碱最终浓度约为13mM)贮存于4℃,历8～10周.测定贮存过程中全血葡萄糖、乳酸和红细胞内酸溶性有机磷及无机磷的含量变化,并与对照比较.结果显示,CDP-胆碱在抑制红细胞溶血的同时,却对全血的葡萄糖消耗、乳酸生成及贮存过程中红细胞内酸溶性有机磷含量的下降、无机磷含量的上升均无影响,表明CDP胆碱抑制溶血的作用与能量代谢基本无关.此外,经测定贮存过程中红细胞去蛋白滤液的紫外光吸收图谱,也看到实验组与对照组完全一致,提示在本实验条件下,CDP-胆碱基本不进入红细胞内部.所有以上结果表明,CDP-胆碱抑制溶血主要不是通过参与细胞内的能量代谢而起作用,因此与嘌呤核苷类物质是完全不同的.根据以上结果,我们认为对CDP-胆碱抑制贮存红细胞溶血机理的研究应着重于细胞膜的结构和功能方面.
It has been previously reported that Cytidine Diphosphocholine (CDP-choline) significantly inhibits the hemolysis of stored erythrocytes. To explore whether the action of CDP-choline is the same as that of purine nucleosides, it is also involved in intracellular energy Metabolism In this article we analyzed some of the biochemical markers of erythrocyte storage, and we measured normal human blood (CDP-choline, final concentration of CDP-choline containing CDP-choline, anticoagulated with acidic citrate dextrose (ACD) 13mM) were stored at 4 ℃ for 8-10 weeks, the content of acid-soluble organic phosphorus and inorganic phosphorus in whole blood glucose, lactate and erythrocytes during storage were measured and compared with the control.The results showed that CDP- Erythrocyte hemolysis at the same time, but the whole blood glucose consumption, lactic acid production and storage of red blood cells within the acid-soluble organic phosphorus content decreased, inorganic phosphorus content increased no effect, indicating that CDP choline inhibition of hemolysis and energy metabolism basic Irrelevant.In addition, after determination of storage process of erythrocyte deproteinized filtrate UV absorption spectra, also see the experimental group and the control group exactly the same, suggesting that under the experimental conditions, CDP-choline is basically not Into the red blood cells inside. All of the above results show that, CDP-choline inhibition of hemolysis is not mainly through the involvement of energy metabolism in the cell and therefore, is different from purine nucleosides. Based on the above results, we believe that CDP- The study of mechanism of alkali inhibition of hemolysis of stored red blood cells should focus on the structure and function of the cell membrane.