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目的探究人类免疫缺陷病毒(HIV)感染者感染1年内HIV-1总DNA、2-LTR环状DNA和整合型DNA的变化情况。方法从HIV急性期队列中选取疾病进展速度不同的两组患者,“高CD4组”CD4计数在1年内大于500个/μl,“低CD4组”CD4计数在1年内小于300个/μl。比较两组患者在同一感染时间点时总HIV DNA、2-LTR环状HIV DNA及整合HIV DNA的水平,及同一组患者在不同感染时间点时的总HIV DNA、2-LTR环状HIV DNA和整合HIV DNA的水平。结果 “高CD4组”和“低CD4组”间总HIV DNA在感染第12个月时差异有统计学意义(U=2.958,P<0.05),2-LTR环状HIV DNA及整合HIV DNA在两组间差异无统计学意义。“高CD4组”总HIV DNA在感染第1个月和第12个月间差异有统计学意义(H=2.502,P<0.05),“低CD4组”各个月份间差异无统计学意义。结论对于急性HIV感染的患者,尤其是感染1年内的患者,总HIV DNA有较好的临床应用价值,可以辅助预测患者的疾病进展情况。
Objective To investigate the changes of HIV-1 total DNA, 2-LTR circular DNA and integrative DNA in human immunodeficiency virus (HIV) infected patients within one year. METHODS: Two groups of patients with different rates of disease progression were selected from the acute phase cohort of HIV. The “high CD4” CD4 count was greater than 500 per 1 μL in 1 year and the “low CD4” CD4 count was less than 300 in 1 year / μl. The levels of total HIV DNA, 2-LTR circular HIV DNA and integrated HIV DNA at the same infection time were compared between the two groups, and the total HIV DNA, 2-LTR circular HIV DNA at different infection times And integrate HIV DNA levels. Results The difference of total HIV DNA between the “high CD4” group and the “low CD4” group at the 12th month of infection was statistically significant (U = 2.958, P <0.05) Integration of HIV DNA was not statistically different between the two groups. There was no significant difference between the “high CD4” and “total HIV DNA” in the first month and the 12th month of infection (H = 2.502, P <0.05) Significance of learning. Conclusion For patients with acute HIV infection, especially in patients infected within 1 year, total HIV DNA has a good clinical value, which can help predict the patient’s disease progression.