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目的：为探讨细胞凋亡在脑缺血再灌注时脑损伤过程中的作用。方法：采用免疫组化法检测大鼠脑缺血再液注不同时间内ｃ－ｆｏｓ、ｂｃｌ－２蛋白表达的水平。结果：①脑缺血再灌注时在缺血侧皮层和基底节区可见ｃ－ｆｏｓ阳性表达，并于缺血 ３０ｍｉｎ再灌注１ｈ时阳性表达达高峰；②脑缺血再灌注时缺血侧皮层和基底节区均有ｂｃｌ－２阳性表达，于缺血２ｈ再灌注３ｈ时阳性表达达高峰。结论：ｃ－ｆｏｓ和ｂｃｌ－２参与大鼠脑缺血再灌注时缺血性细胞损伤的发生。
Objective: To investigate the role of apoptosis in brain injury following cerebral ischemia-reperfusion. Methods: The expression of c-fos and bcl-2 protein was detected by immunohistochemistry in different time after cerebral ischemia and reperfusion in rats. Results: ① The positive expression of c-fos was observed in ischemic cortex and basal ganglia at cerebral ischemia and reperfusion, and peaked at 1h after reperfusion at 30min ischemia. ② The expression of c-fos in ischemic cortex and basal ganglia at ischemia- And basal ganglia were bcl-2-positive expression in ischemia 2h reperfusion 3h positive expression reached its peak. Conclusion: c-fos and bcl-2 are involved in the development of ischemic cell injury during cerebral ischemia-reperfusion in rats.