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正常Wistar鼠87只(雌41只,雄46只)按年龄分为4组:幼年27只(4～6周)、青年20只(16周)、中年20只(26～32周)、老年20只(56～76周)。采用贴块法对鼠主动脉内皮细胞作传代培养,于第2、4、12和20代采样共380份,分别测培养液中6-酮-前列腺素F_1α(6-酮-PGF_(1α)),和血栓素B_2(TXB_2),(T)的含量,同时还观察了四组中各代龄的细胞生长率。结果表明,生命旺盛期(幼、青年组的2、4代)细胞增长率明显高于中老年期,且P较低,T水平较高,T/P值明显增加,有利于细胞增殖,而T/P降低是衰老的表现。
Eighty-seven Wistar rats (41 females and 46 males) were divided into 4 groups according to their age: 27 in young (4-6 weeks), 20 in young (16 weeks), 20 in middle-aged (26-32 weeks) Elderly 20 (56 to 76 weeks). A total of 380 samples were collected on the 2nd, 4th, 12th and 20th passages of the rat aorta endothelial cells. The contents of 6-keto-prostaglandin F_1α (6-keto-PGF_ (1α) ), Thromboxane B 2 (TXB 2) and (T), and the cell growth rates of all age groups were also observed. The results showed that the cell growth rate was significantly higher than that of middle-aged and elderly patients in the period of vigorous life (2,4 and 4 generations of young and youth group), and the P was lower, the T level was higher, the T / P value was obviously increased, T / P reduction is a sign of aging.