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分别在C18柱和MetachemTaxsil 3紫杉醇专用柱上研究了云南红豆杉粗提物中紫杉醇(1)、三尖杉宁碱(2)、7 表 10 去乙酰基紫杉醇(3)和7 表 10 去乙酰基三尖杉宁碱(4)的反相色谱行为.在MetachemTaxsil 3紫杉醇专用柱上,水:乙腈(54:46)的流动相使这四种色谱行为相近的化合物获得了基线分离.使用溴化法处理含有(2)和(4)的红豆杉粗提物,通过分离纯化得到了二溴 7 表 10 去乙酰基三尖杉宁碱的两个非对映异构体(5和6).体外活性实验结果表明,5和6对于人乳腺癌(MCF 7)、人肺癌(A549)和人卵巢癌的体外毒性比紫杉醇略强.
Taxus chinensis (1), cephalomannine (2), 7 deacetyl paclitaxel (3) and 7 were tableted on C18 column and Metachem Taxus 3 paclitaxel column respectively. Reversed Phase Chromatography of Cephalcalyptin (4). The mobile phase of water: acetonitrile (54:46) on the Metachem Taxus 3 column was baseline separated for the four compounds with similar chromatographic behavior. The crude extract of yew (2) and (4) was treated by the chemical method to obtain dibromo 7 by isolation and purification. The two diastereomers of deacetylcornylamine (5 and 6) In vitro activity assay results showed that in vitro toxicity of 5 and 6 was slightly stronger than that of paclitaxel in human breast cancer (MCF 7), human lung cancer (A549) and human ovarian cancer.