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目的：为了探讨胞浆游离钙、ＡＴＰ、ＡＤＰ、ＡＭＰ在脑缺血和再灌流损伤中的作用及相互关系以及药物的影响。方法：应用沙鼠建立脑缺血模型。缺血前１５分钟腹腔注射药物，分别阻断双侧颈动脉５０分钟，阻断颈动脉５０分钟再灌流１０分钟，再灌流６０分钟及１２０分钟。检测脑组织中ＡＴＰ、ＡＤＰ和ＡＭＰ的变化以及应用尼莫地平及东莨菪碱后的影响。结果：（１）胞浆游离钙在脑缺血５０分钟后大幅度升高，再灌流时再度升高，然后缓慢下降。（２）在缺血期ＡＴＰ迅速下降，再灌流期先上升，此后再度下降，而ＡＤＰ及ＡＭＰ在缺血及再灌流期积聚。（３）尼莫地平及东莨菪碱对减缓胞浆游离钙升高，减缓ＡＴＰ的耗竭均有作用。结论：对脑缺血和再灌流损伤有治疗意义。
OBJECTIVE: To investigate the role and relationship of cytosolic free calcium, ATP, ADP and AMP in cerebral ischemia and reperfusion injury and the influence of drugs. Methods: Gerbils were used to establish cerebral ischemia model. 15 minutes before ischemia, intraperitoneal injection of drugs, respectively, blocking the bilateral carotid artery for 50 minutes, blocking the carotid artery for 50 minutes reperfusion 10 minutes, reperfusion 60 minutes and 120 minutes. The changes of ATP, ADP and AMP in brain tissue and the effect of nimodipine and scopolamine were detected. Results: (1) The cytoplasmic free calcium increased significantly after 50 minutes of cerebral ischemia and increased again during reperfusion, then decreased slowly. (2) During the ischemic period, ATP decreased rapidly and then increased at reperfusion stage and then decreased again. However, ADP and AMP accumulated during ischemia and reperfusion. (3) Nimodipine and scopolamine have the effects on slowing the increase of cytosolic free calcium and slowing the depletion of ATP. Conclusion: It is of therapeutic significance to cerebral ischemia and reperfusion injury.