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本文对自制赝复体用硅橡胶材料的体外细胞毒性进行测定与评价,为后期临床应用提供依据。参照国标GB/T16886.5-2003,采用CCK-8法检测新型硅橡胶对小鼠成纤维细胞L929的细胞毒性,计算细胞相对增殖率,通过倒置相差显微镜和扫描电镜观察细胞形态。结果表明新型硅橡胶材料的细胞相对增殖率为91.65%(24h)、87.03%(48h)和87.30%(72h),细胞毒性1级,存在轻微细胞毒性。电镜观察24、48和72h的细胞均呈现典型的长梭形、长三角形形态,细胞生长良好。结果显示,新型硅橡胶材料在细胞毒性方面,符合生物医学材料要求。
In this paper, self-made prosthesis with silicone rubber material in vitro cytotoxicity were measured and evaluated for the late clinical basis. Reference to the national standard GB / T16886.5-2003, the CCK-8 method was used to test the cytotoxicity of the new silicone rubber on the mouse fibroblast L929 cells, the relative cell proliferation rate was calculated, and the cell morphology was observed by inverted phase contrast microscope and scanning electron microscope. The results showed that the relative cell proliferation rates of the new silicone rubber materials were 91.65% (24h), 87.03% (48h) and 87.30% (72h), cytotoxicity grade 1 with slight cytotoxicity. Electron microscopy showed that cells at 24, 48 and 72 hours showed typical long fusiform and long triangle morphology with good cell growth. The results show that the new silicone rubber material in the cytotoxicity, in line with the requirements of biomedical materials.