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应用阳性选择策略，以新型ＩｓｏｌｅｘＴＭ５０系统建立了富集ＣＤ３４＋造血干／祖细胞（ＨＳＣ／ＨＰＣ）的免疫磁珠无菌分选术，对正常骨髓单个核细胞中的ＣＤ３４＋ＨＳＣ／ＨＰＣ进行富集与鉴定。结果表明，富集前ＣＤ３４＋ＨＳＣ／ＨＰＣ为１．４％～３．４％，富集后纯度高达８７．５％～９５．０％，回收率为４９％～８０％，仍保持细胞活力。该系统具有高效、快速和可复性好的特点，每次能分选２×１０９单个核细胞，可获取较多的ＣＤ３４＋ＨＳＣ／ＨＰＣ，整个分离过程≤３．５小时，为深入研究人类ＨＳＣ／ＨＰＣ的生物学特征与功能奠定了基础
The positive selection strategy was used to establish the immunomagnetic separation of CD34 + hematopoietic stem / progenitor cells (HSC / HPC) by the new IsolexTM50 system, and the enrichment and identification of CD34 + HSC / HPC in normal bone marrow mononuclear cells . The results showed that pre-enrichment CD34 + HSC / HPC was 1.4% ~ 3.4%, the enrichment purity was as high as 87.5% ~ 95.0%, the recovery was 49% ~ 80%, still maintain cell viability. The system is characterized by high efficiency, rapidity and reproducibility. Each collection of 2 × 109 mononuclear cells can obtain more CD34 + HSC / HPC. The whole separation process is less than 3.5 hours. To further study the effect of human HSC / HPC biological characteristics and function laid the foundation