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应用高效液相色谱法(HPLC)测定了正常大鼠肺及肝组织中细胞膜磷脂的含量及热应激时膜磷脂的含量变化。流动相为甲醇∶乙腈∶85%磷酸(3∶100∶1,V/V/V),色谱柱为μ-Porasil柱(3.9mmi.d.×300mm)。通过测定膜磷脂的变化,可以为阐明机体的发病机理提供可靠的数据
The content of plasma membrane phospholipid and the content of membrane phospholipid in normal rat lung and liver tissue were measured by HPLC. The mobile phase consisted of methanol: acetonitrile: 85% phosphoric acid (3: 100: 1, V / V / V) and the column was μ-Porasil column (3.9mmi.d × 300mm). By measuring membrane phospholipid changes, we can provide reliable data to elucidate the pathogenesis of the body