论文部分内容阅读
目的以唐德链霉菌Streptomyces tendae ATCC 31160为原始菌株,通过选育获得高产突变株,并研究高产菌株的工业化发酵工艺。方法根据尼可霉素Z的生物合成途径,通过离子束注入方法,选育对关键中间产物2-吡啶-甲醛(PA)和3-甲基天冬氨酸的结构类似物S-(2-氨基乙基)-L-半胱氨酸(AEC)具有耐受性的突变菌株,使突变菌株的代谢能够朝着产物NIK合成的方向。结果最终筛选到一支高产突变菌株,其抗生素产量达到3000mg/L,较出发菌株提高150倍以上。在此基础上,建立了小试发酵工艺路线。结论高产突变株的筛选和发酵工艺的确定为该项目产业化奠定了基础。
OBJECTIVE To obtain high yield mutant strains by Streptomyces tendae ATCC 31160 and to study the industrial fermentation process of high yield strains. Methods According to the biosynthetic pathway of nikkomycin Z, the structural analogues of 2- (2-pyridyl) formalin (PA) and 3-methyl aspartate (S- Aminoethyl) -L-cysteine (AEC) -based mutants, allowing the metabolic changes of the mutant strains in the direction of product NIK synthesis. As a result, a high-yielding mutant strain was finally screened. Its antibiotic production reached 3000mg / L, which was 150 times higher than that of the original strain. On this basis, established a small test fermentation process. Conclusion The selection of high-yielding mutant strains and determination of fermentation process laid the foundation for the industrialization of this project.