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目的研究冬凌草醇提物单次灌胃给药对小鼠的半数致死量(LD50)。方法按Bliss法设计试验,对小鼠单次灌胃,观察14d内小鼠的毒性反应和死亡情况,以小鼠急性死亡率为指标,测定冬凌草醇提物对小鼠LD50和LD50的95%的可信限。结果冬凌草醇提物单次灌胃给药的LD50为1.9923 g/kg,LD50的95%的可信限为1.7213~2.3113g/kg。对死亡小鼠剖解后肉眼观察心、肝、肾、肺、脑、脾、胸腺等重要器官无明显异常发现,但多数动物十二指肠色红充血。结论冬凌草醇提物具有一定的毒性。
Objective To study the half-lethal dose (LD50) of sterol extract of Rubescens militaris to mice by single gavage. Methods According to the Bliss design test, the mice were intragastrically gavaged and the toxicity and mortality of the mice were observed within 14 days. The acute mortality rate of mice was used as the index to determine the inhibitory effect of Rubesternia oxytoca extract on LD50 and LD50 95% confidence limit. Results The LD50 of single administration of Rubescens alcoholic extract was 1.9923 g / kg, and the 95% confidence interval of LD50 was 1.7213 ~ 2.3113 g / kg. No significant abnormalities were found in the vital organs such as heart, liver, kidney, lung, brain, spleen, thymus and so on, which were observed macroscopically after the dead mice were dissected. However, the duodenal red color of most animals was hyperemic. Conclusion Rubescens alcohol extract has some toxicity.