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目的研究心房峡部L型钙电流的特性,探讨心房峡部参与心律失常的机制。方法新西兰大白兔15只,麻醉后取心脏,灌流后分离右心房峡部及游离壁,利用全细胞膜片钳技术,对比观察心房峡部和右心房游离壁L型钙电流的电流密度,并观察异丙肾上腺素灌流后,其电流密度的变化。结果正常状态下,心房峡部和右心房游离壁L型钙电流的电流密度分别为-(7.03±0.71)pA/pF、-(6.30±0.61)pA/pF,二者比较差异无显著意义(P>0.05)。异丙肾上腺素灌流后,心房峡部和右心房游离壁L型钙电流的电流密度分别为-(11.01±0.46)pA/pF、-(8.51±0.31)pA/pF,两处的L型钙电流均显著增大,且心房峡部的L型钙电流密度显著大于右心房游离壁(P<0.01)。结论L型钙电流的异常变化可能是心房峡部参与心律失常发生机制的重要因素。
Objective To study the characteristics of L type calcium current in atrial isthmus and to explore the mechanism of atrial isthmus participating in arrhythmia. Methods Fifteen New Zealand white rabbits were anesthetized and hearts were removed. Right atrium isthmus and free wall were isolated after perfusion. Whole cell patch clamp technique was used to observe the current density of L type calcium current in atrial isthm and right atrium free wall. Adrenaline after perfusion, the current density changes. Results Under normal conditions, the current densities of L-type calcium currents in atrial isthmus and right atrium were - (7.03 ± 0.71) pA / pF, - (6.30 ± 0.61) pA / pF respectively, with no significant difference > 0.05). After isoproterenol infusion, the current densities of L-type calcium currents in atrial isthmus and right atrium were - (11.01 ± 0.46) pA / pF, - (8.51 ± 0.31) pA / pF, respectively Were significantly increased, and atrial isthmic L-type calcium current density was significantly greater than the right atrium free wall (P <0.01). Conclusion The abnormal changes of L-type calcium current may be an important factor of atrial isthmus participating in the mechanism of arrhythmia.